Research progress in high-throughput phenotype testing technology and equipment for engineering cells based on microfluidics

Authors

Shuang LI, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, ChinaFollow
Xiaojie GUO, TMAXTREE Biotechnology Co. Ltd., Luoyang 471023, China
Haibo CHEN, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China
Sisi CHEN, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China
Xin DA, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China
Zhenghui LI, Beijing Key Laboratory of Biomass Waste Resource Utilization, College of Biochemical Engineering, Beijing Union University, Beijing 100023, China
Qinxiu LIU, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China
Yi WANG, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China
Xinhui XING, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China
Chong ZHANG, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaFollow

Keywords

microfluidics; single-cell; high-throughput testing; synthetic biology; biomanufacturing

Document Type

Biomanufacturing: Retrospect and Prospects

Abstract

Engineering cells are a core component of green bio-manufacturing. The efficient testing and acquisition of engineering cells with the desired phenotype is a critical factor in the successful implementation of bio-manufacturing. In recent years, based on microfluidics technologies, non-culture type single-cell phenotype high-throughput test equipment and culture type phenotype high-throughput test equipment for the growth and metabolism of single cell in a bioreactor have been developed such as microdroplet and microchamber, realizing high-throughput, automated, miniaturized, and integrated testing of the phenotypes of engineering cells and providing a powerful tool for the breeding of engineering cells. This review systematically compiles the research progress of non-culture type single-cell phenotype testing technologies and equipment based on fluorescence signals, Raman signals, image signals, and magnetic signals, as well as the droplet and microchamber culture type single-cell test technologies and equipment based on picoliter, nanoliter, and microliter multi-scale microbioreactors, and explores the development trend of the creation of high-throughput testing instruments for engineering cells, providing a reference for the research of green bio-manufacturing engineering cells testing technologies.

First page

91

Last Page

106

Language

Chinese

Publisher

Bulletin of Chinese Academy of Sciences

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Recommended Citation

LI, Shuang; GUO, Xiaojie; CHEN, Haibo; CHEN, Sisi; DA, Xin; LI, Zhenghui; LIU, Qinxiu; WANG, Yi; XING, Xinhui; and ZHANG, Chong (2024) "Research progress in high-throughput phenotype testing technology and equipment for engineering cells based on microfluidics," Bulletin of Chinese Academy of Sciences (Chinese Version): Vol. 40 : Iss. 1 , Article 9.
DOI: https://doi.org/10.16418/j.issn.1000-3045.20241203003
Available at: https://bulletinofcas.researchcommons.org/journal/vol40/iss1/9