Bulletin of Chinese Academy of Sciences (Chinese Version)
synthetic biology; chemicals; cell factories; multi-enzyme molecular machines
Biosynthesis of chemicals through synthetic biology can partially relieve the dependence on petroleum resources. With the development of synthetic biology technology, the capabilities of constructing microbial cell factories for production of chemicals have been greatly improved. Development of new engineered strains and new bioprocess can replace the traditional manufacturing process, decrease the energy cost and pollution, and improve the producing capabilities of traditional fermentation industry. In this review, key factors and representative cases for constructing microbial cell factories and multi-enzyme molecular machines to produce chemicals were summarized. The future of industrial applications of synthetic biology were also discussed.
Bulletin of Chinese Academy of Sciences
McShan D C, Rao S, Shah I. PathMiner:predicting metabolic pathways by heuristic search. Bioinformatics, 2003, 19(13):1692-1698.
Wang H H, Isaacs F J, Carr P A, et al. Programming cells by multiplex genome engineering and accelerated evolution. Nature, 2009, 460(7257):894-898.
Na D, Yoo S M, Chung H, et al. Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature Biotechnology, 2013, 31(2):170-174.
Zhang F, Carothers J M, Keasling J D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature Biotechnology, 2012, 30(4):354.
Lee M J, Mantell J, Hodgson L, et al. Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm. Nature Chemical Biology, 2018, 14(2):142-147.
Dueber J E, Wu G C, Malmirchegini G R, et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nature Biotechnology, 2009, 27(8):753-759.
Badarinarayana V, Estep P W Ⅲ, Shendure J, et al. Selection analyses of insertional mutants using subgenic-resolution arrays. Nature Biotechnology, 2001, 19(11):1060-1065.
Alper H, Moxley J, Nevoigt E, et al. Engineering yeast transcription machinery for improved ethanol tolerance and production. Science, 2006, 314(5805):1565-1568.
Jarboe L R, Zhang X, Wang X, et al. Metabolic engineering for production of biorenewable fuels and chemicals:contributions of synthetic biology. Journal of Biomedicine and Biotechnology, 2010:761042. Doi:10.1155/2010/761042.
Lynch M D, Warnecke T, Gill R T. SCALEs:Multiscale analysis of library enrichment. Nature Methods, 2007, 4(1):87-93.
Charles E N, Gregory M W. Metabolic engineering for the microbial production of 1, 3-propanediol. Current Opinion in Biotechnology, 2003, 14(5):454-459.
Ro D K, Paradise E M, Ouellet M, et al. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006, 440(7086):940-943.
张学礼, 张冬竹.一种高产L-丙氨酸的XZ-A26菌株及构建方法与应用: 中国, ZL201110235159.8, 2014-11-23.
Diao L, Liu Y, Qian F, et al. Construction of fast xylosefermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiae by rational design and adaptive evolution. BMC Biotechnology, 2013, 13(1):110. Doi:10.1186/1472-6750-13-110.
Jiang Y, Liu J, Jiang W, et al. Current status and prospects of industrial bio-production of n-butanol in China. Biotechnology Advances, 2015, 33(7):1493-1501.
Yue G, Sun J, Zheng P, et al. Asparaginic acid kinase Ⅲ mutant and host cells and use thereof: USA, US9896734 B2, 2013-05-16.
Zhang Y P, Evans B R, Mielenz J R, et al. High-yield hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS One, 2007, 2(5):e456.
You C, Shi T, Li Y, et al. An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch. Biotechnology and Bioengineering, 2017, 114(8):1855-1864.
Yan, ZENG; Xingang, ZHAO; and Ju, ZHOU
"Current Situations and Perspectives of Industrial Applications of Synthetic Biology,"
Bulletin of Chinese Academy of Sciences (Chinese Version): Vol. 33
, Article 9.
Available at: https://bulletinofcas.researchcommons.org/journal/vol33/iss11/9