Bulletin of Chinese Academy of Sciences (Chinese Version)
synthetic biology; synthetic genetic circuit; reprogramming; environment adaptation; modularization
Genetic circuits are dynamic regulation systems that control the life of every living organism. With the principles of engineering, synthetic genetic circuits are designed through simplifying and re-programming natural genetic circuits, and even by creating completely new principles that does not exist in nature. Genetic circuits are consisting of a wide variety of components, including genetic switches, oscillators, logic gates, and so on. The development of diverse synthetic circuits not only facilitated the understanding of basic principles of life, but also enabled the rebuilding of natural biological systems which has provided brand new solutions for a broad range of applications including medicine, agriculture, and industrial fermentation. In the last two decades, the design of synthetic genetic circuits has been seen rapid development, but the complexity of intercellular biochemical reactions and signal transductions still poses great challenges for building genetic circuits with more sophisticated functions. Consequently, the pathways toward predictable assembly in cells of microscopic scale and ensuring reliable circuit performance in complex environments would be major subjects that challenge all researchers in this field.
Bulletin of Chinese Academy of Sciences
Nandagopal N, Elowitz M B. Synthetic biology:integrated gene circuits. Science, 2011, 333(6047):1244-1248.
Elowitz M B, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature, 2000, 403(6767):335-338.
Gardner T S, Cantor C R, Collins J J. Construction of a genetic toggle switch in Escherichia coli. Nature, 2000, 403(6767):339-342.
You L, Cox R S, 3rd, Weiss R, et al. Programmed population control by cell-cell communication and regulated killing. Nature, 2004, 428(6985):868-871.
Levskaya A, Chevalier A A, Tabor J J, et al. Engineering Escherichia coli to see light-These smart bacteria 'photograph' a light pattern as a high-definition chemical image. Nature, 2005, 438(7067):441-442.
Anderson J C, Clarke E J, Arkin A P, et al. Environmentally controlled invasion of cancer cells by engineered bacteria. Journal of Molecular Biology, 2006, 355(4):619-627.
Balagadde F K, Song H, Ozaki J, et al. A synthetic Escherichia coli predator-prey ecosystem. Molecular Systems Biolog, 2008, 4:187.
Anderson J C, Voigt C A, Arkin A P. Environmental signal integration by a modular AND gate. Molecular Systems Biology, 2007, 3.
Tigges M, Marquez-Lago T T, Stelling J, et al. A tunable synthetic mammalian oscillator. Nature, 2009, 457(7227):309-312.
Greber D, Fussenegger M. An engineered mammalian band-pass network. Nucleic Acids Research, 2010, 38(18):e174.
Auslander S, Auslander D, Muller M, et al. Programmable singlecell mammalian biocomputers. Nature, 2012, 487(7405):123-127.
Muller M, Auslander S, Spinnler A, et al. Designed cell consortia as fragrance-programmable analog-to-digital converters. Nature Chemical Biology, 2017, 13(3):309-316.
Zhang H Q, Lin M, Shi H D, et al. Programming a Pavlovian-like conditioning circuit in Escherichia coli. Nature Communications, 2014, 5:3102.
Moon T S, Lou C, Tamsir A, et al. Genetic programs constructed from layered logic gates in single cells. Nature, 2012, 491(7423):249-253.
Nielsen A A, Der B S, Shin J, et al. Genetic circuit design automation. Science, 2016, 352(6281):aac7341.
Cagatay T, Turcotte M, Elowitz M B, et al. Architecture-dependent noise discriminates functionally analogous differentiation circuits. Cell, 2009, 139(3):512-522.
Park S H, Zarrinpar A, Lim W A. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science, 2003, 299(5609):1061-1064.
Dodd I B, Shearwin K E, Perkins A J, et al. Cooperativity in long-range gene regulation by the lambda CI repressor. Genes Development, 2004, 18(3):344-354.
Cho J H, Collins J J, Wong W W. Universal chimeric antigen receptors for multiplexed and logical control of T cell responses. Cell, 2018, 173(6):1426-1438.
Gupta A, Reizman I M, Reisch C R, et al. Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit. Nature Biotechnology, 2017, 35(3):273-279.
Stanton B C, Nielsen A A, Tamsir A, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nature Chemical Biology, 2014, 10(2):99-105.
Tan C, Marguet P, You L. Emergent bistability by a growthmodulating positive feedback circuit. Nature Chemical Biology, 2009, 5(11):842-848.
Cookson N A, Mather W H, Danino T, et al. Queueing up for enzymatic processing:correlated signaling through coupled degradation. Molecular Systems Biology, 2011, 7:561.
Chunbo, LOU; Pei, DU; Fankang, MENG; Xiangyu, JI; and Yihao, ZHANG
"Development and Challenges of Synthetic Genetic Circuits,"
Bulletin of Chinese Academy of Sciences (Chinese Version): Vol. 33
, Article 3.
Available at: https://bulletinofcas.researchcommons.org/journal/vol33/iss11/3